(1)英文名稱:HMC3細胞,別稱:CHME-3細胞;CHME3細胞;HMC-3細胞
(2)中文名稱:人小膠質細胞
(3)HMC3細胞:HMC3細胞系通過人類胎兒腦源性原代小膠質細胞培養經SV40T依賴性永生建立。通常被應用于神經科學及神經炎癥的研究。該永生化的小膠質細胞群能無限性生長,用于小膠質細胞的生化功能分析,它們也易于轉染而通常用于轉染基因的表達研究,具有原代小膠質細胞的特性。
(4)基因表達:Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).
(5)組織來源:人腦
(6)生長方式:貼壁生長
(7)細胞形態:巨噬細胞樣
(8)HMC3細胞完全培養基:MEM+10%FBS+1%雙抗
(9)培養條件:37℃,5%CO2
(10)規格:T25方瓶或1ml凍存管
(11)細胞數量:1×10^6
(12)運輸方式:常溫運輸(T25方瓶)或干冰運輸(凍存管)
(13)支原體檢測:陰性
(14)STR profiling
